LOCUS       EY194843                 335 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12g01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194843
VERSION     EY194843.1  GI:159497689
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 529
            PolyA tail found inside read...SR being truncated to 487
            Potential full insert read...vec-to-vec length: 407
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggagc gggttcttcg tcaacgcaat tcgcggattc ggtgccgccc tggtgctggc
       61 gctttacaac gatctgacca agttctacta gcgcccagat caattcgaaa tctctcgttg
      121 agcgaattga ttcgttcagt tgttgctctc atttttccct ttgctttgtg aacgaacaaa
      181 acttctggtc cttcattcgt cgtttgttgt ccttttccag tcccactgca gaactcttag
      241 tcttttccgt tcacaaaaat attcccaaca actttttgct gtttttcatt tcacacattt
      301 ttgccgaata atgttgtaag tatgaatggt ggaga
//