LOCUS EY194841 492 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab12f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to dbj|BAA92346.1| CK2 alpha subunit [Hemicentrotus pulcherrimus], mRNA sequence. ACCESSION EY194841 VERSION EY194841.1 GI:159497687 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 492) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 681 PolyA tail found inside read...SR being truncated to 642 Potential full insert read...vec-to-vec length: 564 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..492 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg ggagcgattt gtgcacacgg aaaaccaaca tctggtcaca ccggaggcaa 61 ttgacttttt ggacaagctt ctccgctacg accatcagga ccggcttaca gccagggagg 121 caatggagca tccatacttc tatccggtgg tggaggagca ccgtcagaaa gcggcagcaa 181 ctgctggaac gaatccaaac aatgtctctt cgaactcgtg ccttgggacg ccgggaacgc 241 ctaccaatgg cctcatgcca tccgggcaag tgctcaacga atactgacga aggaagatga 301 cttctcgcgc ccttcccatc tacccactcc ctcccgccac caccaccact tgtcgtctcc 361 accattctct cttgatggac agaacacgtt ttctttgggt tttgcatgaa ccatttttct 421 ttgtatttct catcaccaca tccatacaca tagtttatta tttcgcatgc aaagtttaaa 481 agttttaccg ca //