LOCUS       EY194831                 480 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194831
VERSION     EY194831.1  GI:159497677
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 673
            PolyA tail found inside read...SR being truncated to 631
            Potential full insert read...vec-to-vec length: 552
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa ggtgcagttg aatcttgaga gcgcggaacg aaaggagaag gctcgcgact
       61 tgcgtgagga cgcgatgcgc actcgcatcg atgcgatgga ggcccagctg ctcgcgaaag
      121 gggcagcaat ggccaaacaa gagagcgact tggaccagtt gcagggcgaa caccaccggc
      181 tggaacgcga aacccagtgc atcatctgct gggaaagcaa gcgtgagaca ctgtttttgc
      241 cgtgttgcca ctttatttgc tgcaagaaat gtgcatcgtt tactcaggag tgctctctct
      301 gtcgtggttc gctgcaagga aaaattgacg tgttccaatc ttaaattggc tccagaccgg
      361 gaatcaacgg gaaaaagaaa attgatgttt ttggatctac acatgtctaa tcatgaacca
      421 cgttgtatta aaatccggaa tcattgtttt cttgttctgc ataaaaatca cttgacacta
//