LOCUS       EY194827                 515 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12e07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAA29174.1| collagen 8E, mRNA sequence.
ACCESSION   EY194827
VERSION     EY194827.1  GI:159497819
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 832 was extended to value 832 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 707
            PolyA tail found inside read...SR being truncated to 666
            Potential full insert read...vec-to-vec length: 587
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg gatctccccc aagtactgcg ccatcgacgg aggagtgttc ttcgaggacg
       61 gcactcggcg ttagacgccg tagtgaagtg gacgatggtg gcagtcgagt atggacatct
      121 ttcttctggt ggctcttcat cgttggaagc agtggatcgt ctgtcttgct cttctgattt
      181 tctcgcatcg actcccctca ctgccaattg ttgcgtcgtc ttcaccagta ctatttacat
      241 cgtcatcgca aacatccaac gtcagctgcc attgccggtc cttctcgtcc ctactctcct
      301 ctcttgacca ctctattttt ccgtttgttg ttcccatttg aaaaaggctc aaaagtcact
      361 ttggatagga aaacacagac aaattctagc tctcctatgt cgttctgcat ggatgacaca
      421 aacttgtgca aatggacaga ccaccaatcc tcgccacaaa ccaccatttt catctcatat
      481 tgtcaatgtc tatgtgtgga ataaaaggga acaga
//