LOCUS EY194825 360 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab12e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAP06410.1| similar to NM_019780 vacuolar protein sorting 29 [Schistosoma japonicum], mRNA sequence. ACCESSION EY194825 VERSION EY194825.1 GI:159497817 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 360) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 881 PolyA tail found inside read...SR being truncated to 510 Potential full insert read...vec-to-vec length: 432 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..360 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa tgtagacgtg ttgatttctg gacatacaca cgaatgtaag gtttatgaga 61 aggcgggggt attttacgtg aatcctggtt ccgctacagg tgcctattcc attatcaaaa 121 atgatgtacc cgcctcattt attgtgctcg acgtacagca agatttgatt gtagcttatc 181 tttatcgatt gctgaacgac gaagtcaagg ttgaacgcgt acagttcagg aagggcaata 241 aatgtactga cgaatgatga aggaataaaa aatagtaatt tattatgaat gcaatgtaaa 301 tgaaattgga cgtgaatttg gtacaatgtg ctgagatcaa ttaaatgcgg ttctatcgga //