LOCUS       EY194822                 378 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194822
VERSION     EY194822.1  GI:159497814
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 567
            PolyA tail found inside read...SR being truncated to 529
            Potential full insert read...vec-to-vec length: 450
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat ggagagtcgg ctgcgttgga agtcaaaatg ctaaaatccc atatgcgggc
       61 ttggccgcac atgttggcgg ttgtagtggc aatagtggtg tgccaagttg gaatcgggct
      121 cacactcgcg gcaacacttg cggcaatcgg attcgcgctc gttactggtg gaacagccaa
      181 attgaagaag gaaattgcgg gcttggcgag cgaggtcgct caactgaaac gctgaggaag
      241 atgtcggtcg aaccaaaaga agtaaggcat tcgacttgcc gattgctgtt gaccaatccg
      301 acaattccga tatctcagct tttatgctat tttatttggg atctttgact ccatcttgtt
      361 actttaaatt tggatata
//