LOCUS       EY194821                 582 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12e01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|P30163|ACT2_ONCVO ACTIN 2 gb|AAA29410.1| actin 2,
            mRNA sequence.
ACCESSION   EY194821
VERSION     EY194821.1  GI:159497813
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 841
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcct gaagtacccg attgagcacg gcgtcgtcac caactgggac gacatggaga
       61 agatctggca ccacactttc tacaacgagc tgcgtgttgc ccctgaggag cacccagtgc
      121 tgctgactga ggcaccgctg aacccgaagg ccgaccgtga gaagatgacc cagatcatgt
      181 tcgagacctt caacacgccg gcaatgtacg tggccatcca agccgtgctc tcgctctatg
      241 cgtccggacg taccaccggc attgtgctcg actctggcga tggtgtgacc cacaccgtgc
      301 ccatctacga gggttacgcg ctgccgcacg ccatcctccg tctggacttg gccggccgtg
      361 atctgaccga ctacttgatg aagatcctca ctgagcgcgg ttactcgttc accaccaccg
      421 ccgagcgtga gatcgtccgc gacataaagg agaagctctg ctacgtggca cttgactttg
      481 agcaggagat ggccaccgcc gcctcctctt cgtcgctgga gaagagctac gagttgcccg
      541 acggacaggt gatcaccgtc ggaaacgagc gcttccgttg tc
//