LOCUS       EY194818                 285 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194818
VERSION     EY194818.1  GI:159497810
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 812 was extended to value 813 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 476
            PolyA tail found inside read...SR being truncated to 436
            Potential full insert read...vec-to-vec length: 357
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga actgattttc caagagaccg agaacttcca cctaagagtg accggcgcaa
       61 tggcggcatc caacatcgca cagcagacga gtatggtgga atagccgacg agacttggag
      121 atttttgaat gccattctca ttcttcaatt tctaatctgg aaccaaaata atcacaatat
      181 tttatctatt tagaattaag tgatgatatt tcgtgcccca gattgcatca ctcttattgt
      241 aatagaccga atatgtttga aatatttgaa aaaactctgg tttca
//