LOCUS       EY194817                 496 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12d08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_495019.1| Masculinisation Of Germline 5, sex
            determination DEAH box protein, posttranscriptional regulatory
            factor similar to pre-mRNA splicing factor ATP-dependent RNA
            helicase (135.8 kD) (mog-5) [Caenorhabditis elegans]
            sp|Q09530|MOG5_CAEEL, mRNA sequence.
ACCESSION   EY194817
VERSION     EY194817.1  GI:159497809
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 829 was extended to value 829 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 687
            PolyA tail found inside read...SR being truncated to 646
            Potential full insert read...vec-to-vec length: 568
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcat caagagcgcc ggtagagaca cccagttggt gcagaaagcg atttgctccg
       61 ggttcttccg caatgcagtg aagcgcgatc cccaagaggg ctacaggaca atcgtggacg
      121 gacaaactgt gtacatacat ccctcgagtt ctctgttcca aaatcaaccc gaatgggtgg
      181 tctaccacga actggtccaa acaacaaagg aatacatgcg agaggtcacg acgatagagc
      241 caaactggct ggtggagttc gctccctcct acttcaggaa gagtgacaac accaaactgt
      301 cggcgttcaa gaagaaccag aagatcgacc cgctcttcaa caagtacgag gatccgaatg
      361 cgtggcgtct atcgcggctc aagaagaaaa tctacaaccc gaacagataa ttggttgttt
      421 ttactgcgcg gacatttcca attcattctg tgtaatattt ttcaccgcaa atattttaat
      481 tttggaatac aatcga
//