LOCUS       EY194815                 545 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12d06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497347.1| Putative cytoplasmic protein of
            bilaterial origin [Caenorhabditis elegans] sp|Q95Y71|RM45_CAEEL
            Putative mitochondrial 39S ribosomal protein L45 gb|AAK29715.2|
            Hypothetical protein Y119D3B.16 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY194815
VERSION     EY194815.1  GI:159497807
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 859 was extended to value 859 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 732
            PolyA tail found inside read...SR being truncated to 695
            Potential full insert read...vec-to-vec length: 617
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggacc gaaacggcat ttgctaaatt gtggccagag gttgagaaag cgacgctcca
       61 atggaagttg gtgaggttca tcgagccctc tcgcgttgtt aacgtccggt gttatgataa
      121 cccccagaag tcgggcaacg acatcgccca aataacggtc cgaatgcaca gcactcagat
      181 tatggccctt tacgacaagg atggacactt gctggtcggg cacccggagg aaacgagcga
      241 ttctctggag tatgtggtgt tcgagaacca cattgccagc ctggatggga aatggcgctt
      301 ccatgacaga atcttcccgg attggctcag agcgaggcag cccatcctca gaaaggggct
      361 aattgagggc aaagacgagg aagagggggc accagtgaaa ctccaggggg gcatgcagac
      421 gttgatagcg gaaatggcca agaaaaggga cgagaaggag aaaaagggaa tgctgcccaa
      481 agacgagtga cgcctagttg ttggttgaat ttttgattgt ttccaaaaat aagatagata
      541 tttca
//