LOCUS       EY194814                 578 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_510139.1| Putative secreted or extracellular
            protein family member precursor of eukaryotic origin (22.1 kD)
            [Caenorhabditis elegans] pir|T20159 hypothetical protein C52G5.2 -
            Caenorhabditis elegans emb|CAA91797.1| Hypothetical protein C52G5,
            mRNA sequence.
ACCESSION   EY194814
VERSION     EY194814.1  GI:159497806
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 644 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 793
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ctggattgca aatggcgctc cgaattctct ggtaaagtgc aatggcccag
       61 cattccaaca atgacgttcg gcgaagagct gctgattgcc gaggcaccag tcgccaaaac
      121 gtccgaagtc caattcctca attggagcgc tcgcgcttgg tcccattcca ccaaggacca
      181 tttccacgat gaatggggct tcattactgt ggacgggaac ggaaatgcga ccctgatgac
      241 ggcaggaaac aatggcttca caacttacga ggtgggcgag gtgcgcaaca acaaaatggt
      301 gctggtcctg aaggacattg gacgcatctc tttctcgagg gatttgcccg tggaggactt
      361 gcgccgaaca ttcatcaaac acgacgacac gtatctggaa cagatcctcg agatgcgaac
      421 agcaacgcat ccccgaggcg gataccttga acacacgcga gtgatctaca cgcgaatgaa
      481 cacagccatc tgaagaaaac ggatgaatct aaaatagtac gtgtgtccaa tttgtatgac
      541 aaaacattta aatttattcg accagaaggc cgctctgg
//