LOCUS       EY194800                 675 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12c01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_502317.1| Proline HYdroxylase PHY-2 (61.5 kD)
            (phy-2) [Caenorhabditis elegans] sp|Q20065|P4H2_CAEEL Prolyl
            4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2)
            (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit)
            pir|T21816, mRNA sequence.
ACCESSION   EY194800
VERSION     EY194800.1  GI:159497792
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 810
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggct gggctaaagg atgtcagtgt gttcaacgcc caaacgggca gcacaatggt
       61 agtgcccgac aggatcagtc aatccgcctg gcttcccggc tcaatgcatc cgctcatcgt
      121 gcacattaac aaacggcttt cggaaatcgc caatctcgat tggagaacgg cggaacactt
      181 acaagtggcc agctacggca tcggaggcta ctaccatccg caccacgatt tctacacggg
      241 cgactcttta cctcccaagt ttgtggaaaa ggggaatcga attgccacgg caatgattta
      301 tatgtcccgg ccgaggaaag gcggtcgcac tgcattcccc gaactggacc tgagtgtcca
      361 gccgtccgtt ggcgatgtac tgttctggta caacctgcag ccggacggga accccgactt
      421 cagaacactg catgccgcat gcccggtgct aactggccac aagtgggtct ccaacaagtg
      481 gttccacgag cgcggacagg agttcacgcg accttgccca attgcgcatg cgcaacatac
      541 gcagctgttc tgaattacgc gagaaaaact gttttggaga gagtttttgt catgtccgcg
      601 atacaccgaa tgcagtatat ttattgtttg taccttttgc gtagatttct gtttggggaa
      661 cgaaaaaaaa aaaaa
//