LOCUS       EY194779                 545 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|Q23400|RCL1_CAEEL Probable RNA 3'-terminal phosphate
            cyclase-like protein gb|AAB93426.2| Hypothetical protein ZK1127.5
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY194779
VERSION     EY194779.1  GI:159497771
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 820
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc gaatatgaag tggactgtgg cacttcgcgc tccattatct attttctcga
       61 accgctcatc ttcctagcgc ccttttgcaa gaaaccgttg agtgttcgcc ttaagggcgt
      121 caccaatgct ccagatgagt tgagtgtgga cactataaga gcgacctggt tgcctgtatt
      181 ttcccgcttt gttttggcgg atcaaagtga cgaaataaag atagttgcgc gtggtttttt
      241 gcctgatgga ggcggagtgg tgacattcaa cagtccaata aagcgcagct tgcgctctat
      301 tcaatgtgtt aggccaggga aaatatgcaa ggttcgaggt cttgcctacg tttgcaaagt
      361 atctccatca attgcatcca gaatgattga gggggcgaaa aaaatgcttc atggctatat
      421 cgcggatgtt tatgtcacag tggatcaaag aaaaggcgcc caaggtggac gatcaccagg
      481 ctttggaatt tttctgacag cggagactac ggaagggact ttccaccacg gagaggcaat
      541 gtccg
//