LOCUS EY194773 574 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab10h06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_495707.1| Ribosomal Protein, Large subunit RPL-10 (24.7 kD) (rpl-10) [Caenorhabditis elegans] sp|Q09533|RL10_CAEEL 60S ribosomal protein L10 (QM protein homolog) pir|T20688 hypothetical protein F10B5.1 - Caenorhabditis elegans emb|CAA88308, mRNA sequence. ACCESSION EY194773 VERSION EY194773.1 GI:159497765 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 574) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 802 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..574 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga ggtgaaacaa attcaatttt atttttttat agaggctaac aaaccaactg 61 aaaattgatt atgggaaggc gtccggcacg ctgttaccga tacatcaaga acaagccgta 121 cccgaagtca cggttttgtc gtggtgtgcc tgacccaaag atccgaatct tcgatctggg 181 ccgaaaacgc gccaccgtcg acgaatttcc atgctgcgtg catatgattt ccaacgagcg 241 tgaaaatcta tcgtctgagg cgcttgaagc cgcacgtatt tgtgcaaaca aatacatggt 301 caagaattgc ggaaaagatg gcttccacat gcgtgtccga attcatccgt tccacgtgct 361 tcgcatcaac aaaatgttat catgcgccgg cgctgaccgc cttcagacgg ggatgcgagg 421 ggcctttgga aaaccgcaag ggcttgtggc ccgtgtcgga atcggcgatg tgctgatgtc 481 agtgcgtgtc cgtgaccaac acgaggctca tgctctggag gctttccgac gtgcaaagtt 541 caagttcccg gggcgccagt acattgtcgt gtcg //