LOCUS       EY194761                 227 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10g02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194761
VERSION     EY194761.1  GI:159497753
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 775 was extended to value 780 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 424
            PolyA tail found inside read...SR being truncated to 379
            Potential full insert read...vec-to-vec length: 299
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 gggcgggggg cctggacgcg agttccgtga ccaagctggt cattctgccc gccgactcga
       61 agcagctgca cacggtggtc aagggcgaac tggagaagga ggccgaatcc tgaaaggccc
      121 tggggaggca aaaccatagc aagtggttta cctatttagc aacaggttca cacctccaat
      181 tgcgatttgg gcgccatttc ttttcttcta ataaaaaaac tccccta
//