LOCUS       EY194758                 523 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194758
VERSION     EY194758.1  GI:159495992
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 564 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 712
            PolyA tail found inside read...SR being truncated to 674
            Potential full insert read...vec-to-vec length: 595
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca ccaaactccg ccgagcaaag gcgttttggc gaatgcacaa agtggaggca
       61 gaggaacggc acagggcgga agtggcagac accgggagga cttcaaaagc accattttga
      121 aaatggcgag agcaatttgc gcctttgccg aggcggagct ttgagacgcc caaaacgagg
      181 gaaccattta aaatttaaaa ctgaattttt actatttaat tttgcctaaa tttgccattt
      241 ttctttttta atgatcgatt tttgactcaa ttttctattc gattttgatt tcaccgctgc
      301 aaacagggtt tggcacaaac aattgaattg tctccgtgaa tttgttggaa atcgggaagt
      361 ctaataattt tcacaccgat gcctgttccc actgttgttt gttaatttat ttattctctg
      421 tcggatcgcg agcaaacaaa tcgttcctaa tatattttat ttatcagagc gcagataatt
      481 tgtaatttac taatttattt tggataaacg agttttaact tta
//