LOCUS       EY194751                 460 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194751
VERSION     EY194751.1  GI:159495985
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 824 was extended to value 825 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 653
            PolyA tail found inside read...SR being truncated to 611
            Potential full insert read...vec-to-vec length: 532
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggctc caacaccagc caatacaact cgttcaaaaa ctccatcatc aactgcatca
       61 atggactaat cggcaataac ccgaccgtgg aggactgctg catctactac atcaacggat
      121 tatacccatc caacgggggc agccaaaccc tcgccatgcg caaaacgttc ctcgcgacgc
      181 cgttcttcac ctattacacg caggagacta cagtgagcat ccagatcgcg actattagca
      241 tcttctgtgg ctgcaattga ggcgcaagac agaagagagt ggcgtccaac ggattccttt
      301 ctcgaggaag tcaccacgtg ttgagccgca ccttttctct ccagcacttt cctgtgcata
      361 gtcctaaaag aggcaagaca tcgccctttg acccaaaacg acagaacacc gccgcttttt
      421 gtagttttct tacactttcc ttgtgaataa atggtgcgta
//