LOCUS       EY194749                 316 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194749
VERSION     EY194749.1  GI:159495983
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 786 was extended to value 786 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 546
            PolyA tail found inside read...SR being truncated to 467
            Potential full insert read...vec-to-vec length: 388
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc acagtggtgg gtgcgcaatg ggggagaagg gagcgcagag actgacgaat
       61 cggacgacca ggaggagtac gtctggagca ttctgtacga cattttgcat tcggaagggg
      121 tgcacgacgc gttggaaccg gagatgatgg aagagctggt gaaggtggtt caagcgaaca
      181 aaccgagtac tatgccctag tgagagggct cgatcaaacc gcatgggctc tctgttgatt
      241 attcaagcca aaaaggcctc ctctgttgag cagaacatgt ttattttttg aaatggcgat
      301 aaacacgaag caatga
//