LOCUS       EY194745                 467 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10e09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_502065.1| Ubiquitin conjugating enzyme UBC-2,
            LEThal LET-70 (16.7 kD) (let-70) [Caenorhabditis elegans]
            sp|P35129|UBC2_CAEEL Ubiquitin-conjugating enzyme E2-17 kDa
            (Ubiquitin-protein ligase) (Ubiquitin carrier protein) pir|T23820
            hypothetic, mRNA sequence.
ACCESSION   EY194745
VERSION     EY194745.1  GI:159495979
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 793 was extended to value 794 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 681
            Potential full insert read...vec-to-vec length: 466
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggctc cctaccaagg cggcgttttc ttcctcacca tccactttcc tactgattac
       61 ccgttcaaac cgcccaaagt tgcttttacc actcgcatct accatccgaa tatcaacagc
      121 aacgggagca tttgcttgga tattcttaga tctcagtggt ctcccgcact gaccatctca
      181 aaagttctgc tctcgatctg ctcacttctc tgtgatccga acccggacga cccattggtg
      241 ccagagattg cgcgcatcta caagaccgat cgcgagagat acaacacact tgcacgggaa
      301 tggactcaga agtatgctgt gtaacgggca cggccgggat aaagcgaagg ctagtggtgg
      361 atatttgaaa tggcagatgg atgtttggat ctttttatga cgcggcaaat ttgacaatcc
      421 gttttttcat ccatttctcg acttatatta aataaaaatg aaggaga
//