LOCUS       EY194744                 340 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194744
VERSION     EY194744.1  GI:159495978
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 535
            PolyA tail found inside read...SR being truncated to 492
            Potential full insert read...vec-to-vec length: 412
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaat tcatcgaaaa acgctaaaca tttttcccga gatagcggtg catagcaaaa
       61 actgtctttt gcgattattc cgatttccat tttttaaaaa gacccattca tttctattct
      121 ttctgtgttg tacgactaaa atatccgtca caactgccgc aaaattacat atttacttgg
      181 accttatttt gcggtgtccc aaatttctct gccgttgaca aatccgattt gcttgctatt
      241 ttgattatat ttttggaata cgctatgcat cagatgcttt ccacatttat tcatttctcg
      301 aagcctcgga cacttttatc tgatgataaa tctttaccta
//