LOCUS EY194741 389 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab10e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194741 VERSION EY194741.1 GI:159495975 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 389) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 582 PolyA tail found inside read...SR being truncated to 540 Potential full insert read...vec-to-vec length: 461 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..389 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa ccgtgggcag ttcattgctg cgacggtctt catcgggata cagatggtcc 61 tgttcatctg ggattaccaa attctgcacg gacaggcata caactgagga gacgaggatg 121 gaccccaaac tgggaaatgt ttttggatcg tgtaatgtac cgtcgcacca accaacctat 181 atatcccctc tccaattctt cccatcctac gcttattctc attatcagct aaacttaagc 241 acctctaacc aatcacacca aacaattttg gctttttggc atccgactgc cagtctctct 301 ctctctcttt gcatcgtcca aagtccaatt ttccaaaata tttgtaactg tccctttctt 361 gctgacaaat taaatctgta ctagtgcga //