LOCUS EY194738 367 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab10e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194738 VERSION EY194738.1 GI:159495972 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 367) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 813 PolyA tail found inside read...SR being truncated to 518 Potential full insert read...vec-to-vec length: 439 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..367 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga cgaggaggcg ggggagacac tggcccaggt gctgcagcga ctcactgccc 61 gacacgaaga aaaggccaag gtgcgcgagt tcggccacca gggaccgatg gcgaccatct 121 tcgacgtgaa gttcccgtcg agcttcgaga tgtgagggca atagaagcac agtgttcgga 181 tctccgcaaa aaaaacaaag aacgaccagt cgaatcactt aatttgtttt gttcacttcg 241 aacttaatta atactataga actcagcaat atcgtcgtgt tgtgcttggc cattccagac 301 ggacttaata ctttcattgc tattataatg ttgagtgtga ataactgaat aaaattgttt 361 tttttca //