LOCUS EY194736 342 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab10d12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194736 VERSION EY194736.1 GI:159495970 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 342) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 754 was extended to value 754 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 764 PolyA tail found inside read...SR being truncated to 493 Potential full insert read...vec-to-vec length: 414 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..342 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac aaattttcac acttttccat cttattttgc cttctactgt tcacccaatc 61 aactcaaacc atgctcataa gctttccacc accgaactcg gcgcgttttc gcggctacgg 121 attcgaggga ttttgacaca acggcggtgc actgataatt ttacaccaag aatctgtgca 181 aacacgtgaa gcgccaagat aagatttctt gacctgacgc caaacaaatg ttggacaatg 241 aaagaaagtg aaacaaatct ttattcgatt aattgaatca atttccgttg agcatgtaat 301 gtttttcgaa atacttatgg attatggaca ctcacttggc ca //