LOCUS       EY194734                 467 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194734
VERSION     EY194734.1  GI:159495968
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 660
            PolyA tail found inside read...SR being truncated to 618
            Potential full insert read...vec-to-vec length: 539
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa agcatatttg ccagcgctga ttcgcttcgt agaactttgt ggaccagcaa
       61 aattgatgca atgcttcagc ggagcagttg aaaaggagcc agagtgtcac agactcgcac
      121 atcgatgtgc cgtagcggca gttgcgctga agaacagcta ttccttggaa gtgctgaacg
      181 taaacgctga ttttcgacgc gtgctccaat gcccagaaga aatgcaaaaa ttgttgctgg
      241 acaaaagcga cccgacaaaa tggaccgaaa cgctcgaatt tctgaagaat tgacttgact
      301 gacaatgacg cctctttgcc gaacttaaat catttgctag gttcttgcga ttgctttatt
      361 tcttctgacg atatacatac tattcatatt gagtattgac gaaaaatatt tttcttttag
      421 caagcaccaa gtttacaaca aaaatcgata aaatgatgaa gtgcgca
//