LOCUS       EY194727                 449 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194727
VERSION     EY194727.1  GI:159495961
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 811 was extended to value 811 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 642
            PolyA tail found inside read...SR being truncated to 600
            Potential full insert read...vec-to-vec length: 521
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaac aatatgggat tgtgcctggg gccatgattc gcgattcttt gcgactgctt
       61 cgcgggacaa actcttgaat atttggcgga caggaagtga cggagaggtc cccaggcact
      121 ccgcgaagtt cgccagtgga gaaccacaaa ctgctgtaga ttttgcgcca aagcacctgg
      181 cggaagagtg ctctgcctac ttgatcgccc ttggattaga aaatggattt atcctcttgt
      241 tgaattttgt cgcctccacc ggcgaactca gcgaaatcgc ccgactcccc caagaagtga
      301 cccattccag aacgctgact agattgaagt ttcgcccgag tgcggctatc agtgctgaaa
      361 aggagttcca gcttgcttcg tccagcaccg accatttcgt taaaattcac cgaatagaac
      421 tttgttgatt ttagataaat tgttatgca
//