LOCUS       EY194716                 547 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10c03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_060755.1| chromosome 15 open reading frame 12;
            mitochondrial ribosomal protein S4 [Homo sapiens] dbj|BAA91929.1|
            unnamed protein product [Homo sapiens] emb|CAB66847.1| hypothetical
            protein [Homo sapiens] gb|AAH06487.1|AAH06487 hypothetical, mRNA
            sequence.
ACCESSION   EY194716
VERSION     EY194716.1  GI:159495950
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 801 was extended to value 801 (,)
            putative full insert read: vector-to-vector length is 614 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 764
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca cgagaacaag gttgggcgtt agaatgatga tttgcgcggg gaactggtcg
       61 ttttcttttg agataatgcg gcgctaccac gtggaaaaga gggagcacta caccttgtac
      121 aacaaattgt cgcgacaggt ccgcgagttg acaaagagga tccgggattt ggacgacagc
      181 aaccggaaca agttggccct cgcgaggcag ctcctcggca aactctacga cctgggcatt
      241 atccagacgg cggacacgct ggagcggtgc gacagagtca gtgcctcttc gttctgcaaa
      301 agacgactcc ccgtcgttct cttcaaaatg caaatggcgc caagtgtgaa gaaggcgagt
      361 gaactggtgg aacaggggca cgtgagggtc ggcgtcgaga tggtcaccga ccccgcgttt
      421 ctggtcacca ggacgcagtc cgaccacatc acctggacga gggggtcgaa ggtgaagaag
      481 catgtgctcg cctacaacaa cgaactggat gatttcgacg attagccgaa aggccgtaga
      541 attgttg
//