LOCUS       EY194714                 571 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10c01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194714
VERSION     EY194714.1  GI:159495948
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 791 was extended to value 792 (,)
            putative full insert read: vector-to-vector length is 616 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 766
            PolyA tail found inside read...SR being truncated to 722
            Potential full insert read...vec-to-vec length: 643
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga gaacaagcaa cccgtgaagt caacgagcga cgaatggatt caggcccatc
       61 tgcgacgttc gacgcggaac aagaaagtgc cagaacattt tcagccggac atatcgatta
      121 ctcaccgcgc aactctatct cgctccagtt cgcccgatag gtcgagagcg cagacaccca
      181 ctacgcctaa aaggacgcaa caaaagtcag caccaaagga gcccgcttct ccgaaaagac
      241 gacaacgcaa ttccgtcacg ccaccggcga aaacgaatgc caaagggcaa gagcgaactc
      301 ctccgccgtc aacgcgaaag cgcgtcaacc catcgccagc gccgtccacg gccagtaaac
      361 aggcgagagg aagcgaggtg ccccaattag cgaagaagaa agggcgtcag acgaaggaaa
      421 agtgaacgcg ccattgacca tatcgaacat gatccctgta aaagacactt aatacacgta
      481 ttgctctact cttgactctc gccattacat gactacttaa aattgatgct tgttattaat
      541 caaagacgat aaaataaaaa tgccgcctac a
//