LOCUS       EY194705                 326 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab10b04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194705
VERSION     EY194705.1  GI:159495939
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 804 was extended to value 805 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 519
            PolyA tail found inside read...SR being truncated to 478
            Potential full insert read...vec-to-vec length: 398
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga gtcggtgtcg cgatgatgat ttttgttgtc ggctttcttt acgtgaaatt
       61 cgagggaatc accagcaaaa tcgatggcaa attcgagggg gtcaacagca atatcgaggg
      121 ggtcaccatc caattgcagg aaattcgcaa acatcagatt ccctgactgt gctttggaat
      181 agaagtcgtt ggatgaactc ggaattgact tgcacataat gactaagagc tctactattt
      241 cacttaaata catgttcatt tatatcccag actaattaat cacaattctc ctttcatacg
      301 agtgtttaag gaaggtataa cgaata
//