LOCUS EY194674 661 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab09g07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_506222.1| RNA recognition motif containing protein [Caenorhabditis elegans] pir|T24666 hypothetical protein T07F10.3 - Caenorhabditis elegans emb|CAB01243.1| Hypothetical protein T07F10.3 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY194674 VERSION EY194674.1 GI:159498180 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 661) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 824 was extended to value 825 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 828 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..661 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac atgaagatgg gtcatccaaa gagcagggct ttgtgcgttt cacttctgaa 61 actgatcaac agcgtgcatt ggtggaattg aacaaacaca agtttcataa cagagaactc 121 tttctcaaac tggctagacc caaggcgcgt ggtggaccta acgagcgaag atatggaggc 181 ggccatggtg gtcaaggttt acttggtcct ggttatccaa atgcgggtgg atttgcgccg 241 atgcaaacaa tgccgatgat ggggacggtg ccctatggaa tggcaggtac agcgatggct 301 cttgcaactc catacggcgc acaattggcg cctaatcatg gaatggccgg ttttggcagt 361 ccggccactg gacaacagtt cggtggacag ccgttgatgg ttggcaatgc gtatccgcca 421 aatgccttca tgcatccgtt tcaacctgct catatcattc atcccgggcc aatgactccc 481 attggtgggc ctgttccagt tattccaatg aaccagacac tcccggtcaa gttggatgtt 541 cccatccaat acgaagagat cgaacccctc tctccagagg agcacaatgc tattctctac 601 tattcgagcg ctgagtttca cacttttttg gaggcgagcc gttggaacgc gggttgggtt 661 t //