LOCUS       EY194668                 386 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab09f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194668
VERSION     EY194668.1  GI:159498174
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 786 was extended to value 787 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 610
            PolyA tail found inside read...SR being truncated to 537
            Potential full insert read...vec-to-vec length: 458
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcag cgccttctgg tctcgctgtg caacgggtcc agagacgaga gttggcagat
       61 ggtcgcaaag agaggaggac agtcggtggc cgacggagag gagcagagtc cctttgccct
      121 gcaagcgggc agattcgcca ggaatgtgcc gggagagcgg gccggcaccg agaacttcgc
      181 atttgcgttc gccaggaggg acggaaggtt cgccaagagg agcggaggac gaccgtttgc
      241 agtcgagctg attgaagtct tgccagtcga gcagttcgcc aagaggggca aattcgcatg
      301 atgcaacccc aacaaagagg gaatttaccg aataatattt gttcggattg tcggtttaag
      361 accgaaaaat ttcgattcta tgctta
//