LOCUS       EY194657                 510 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab09f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194657
VERSION     EY194657.1  GI:159498154
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 803 was extended to value 804 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 698
            PolyA tail found inside read...SR being truncated to 661
            Potential full insert read...vec-to-vec length: 582
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa tacaaagaaa tcaaagacca actcaaatgg caattgattc cgaaaaaaag
       61 gaatacgaca tcatactgaa ttttatcgcg tctggagttg atgtcaaagc aagtgaaagt
      121 gaaagcaaac aaacactcta caattggaaa aaaagataca ctgtccagct gattggtgac
      181 aaacagcgct tgtttcacgg caagaaggaa gtgatcttct atgagcagct atacgacttg
      241 ctgcacagat cgcatgtgga gaggactggc cacggtggtc gcgatgcaat gaaaaaagat
      301 ttggctgact attatggcct tggacaacgc ataatcatta cttacctcaa aggctgcgaa
      361 gagtgcgaat tgaagaaggc ccgtgtccgc aaatcggtgg tcatcaattt tgttgaatgg
      421 aaaccaaaga aataaatcta gcagtcactt tgtaattctc aatatccaca tatttgcttt
      481 gtttccggca tagaaatggt gatcgcgtta
//