LOCUS EY194652 232 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab09e07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|P90702|RL44_BRUMA 60S ribosomal protein L44 gb|AAC47627.1| ribosomal protein L44 [Brugia malayi], mRNA sequence. ACCESSION EY194652 VERSION EY194652.1 GI:159498141 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 232) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 812 was extended to value 815 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 425 PolyA tail found inside read...SR being truncated to 383 Potential full insert read...vec-to-vec length: 304 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..232 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga gtgcgtggag tgcaaacatc gcatccagaa ggcgatcaag cgctgcaagc 61 actttgagct gggcggacaa aagaagccga agggacaggt catccagttc tgacctgcag 121 ctgggaaaaa agaagaagtg gcggccatca ttttccatct gacaccacaa gagtccccct 181 cccccatact attgtggttg tccgttaaat tatctcgtaa acaaaaaagg ca //