LOCUS       EY194646                 402 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab09e01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194646
VERSION     EY194646.1  GI:159498127
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 822 was extended to value 822 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 597
            PolyA tail found inside read...SR being truncated to 554
            Potential full insert read...vec-to-vec length: 474
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga ggatcagggt tcgactccgg agaaggggcc tgagaaatgg ccactatgtc
       61 taaggatagc agcaggcgcg caaattaccc actctcagca tgaggaggta gtgacgagaa
      121 ataacgaggc cgttctcata cgaggccggc cattggaatg gaagaggttt aaaaccccca
      181 ttgagtatct acgagagggc aagtcaggtg ccagcagccg cggtaattcc agctctcgaa
      241 atgcatagaa ccattgctgc ggttaaaaag ctcgtagttg gacttccgtc gcaccaccgg
      301 tccatctccg gatgcgcact ggtcggcgtg gcttttggcc ggtttcatct ggcagttggc
      361 ttcgctgtcg gctgtcaggg actggcgaat ttaccttgaa ca
//