LOCUS       EY194641                 390 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab09d07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_005326.1| hematopoietic cell-specific Lyn
            substrate 1 [Homo sapiens] sp|P14317|HS1_HUMAN Hematopoietic
            lineage cell specific protein (Hematopoietic cell-specific LYN
            substrate 1) (LCKBP1) pir|S07633 hematopoietic lineage
            cell-specific prote, mRNA sequence.
ACCESSION   EY194641
VERSION     EY194641.1  GI:159498122
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 825 was extended to value 825 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 584
            PolyA tail found inside read...SR being truncated to 542
            Potential full insert read...vec-to-vec length: 462
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag atgccgctag cggtggaaga gcagcagcac caggcccaag ctgtggaggc
       61 gaatccgccg gcagacccgg ccccgtcggt cggactgagt gcggtggcca ttttcgagta
      121 cgagcagcag gacagcgacg aaatcggctt cgacgtcaac gacgtgatca ccgacattga
      181 gcagattgac gccggctggt ggcgcggggt gtgcaaaggg cgctacggac tgttcccggc
      241 caactacgta cagctgatcg aacgacagtg aacacagccg gggatgggcc aaatgacacc
      301 acttttttta aagaatgcct taaccaccta atcatttcta aattttgctt aaaccacaac
      361 ctgttgcgga atataaactc ttaattttga
//