LOCUS       EY194639                 476 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab09d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194639
VERSION     EY194639.1  GI:159498110
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 671
            PolyA tail found inside read...SR being truncated to 627
            Potential full insert read...vec-to-vec length: 548
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggctc ttctggctgt ccacacatcc cctcttttct ccccattcat tttttgttgt
       61 gatgatgcct cgtctcccaa cacgctggat gccttccacg tcccgatccc ttcacaattt
      121 tttgagcttt ttggttgttt cggcgggcgc actcctcaca cacttttccc ttcatgcgca
      181 catttcttca cctgacaccg atttcaataa acctctcccc gtctgaaacc taacttaaca
      241 aaacacatag tacctattta tcgattggtt gtacgttttt atattacata tatgttgcat
      301 ctgttgaccg aaaaagtaat tttgttctga aaatatttcg caaattgatc ttttctcctg
      361 tttctcgtaa ctaacgttgt tgatattcaa actactcaca tccgatcgat caagtgttgt
      421 agtatttaac gtatcagacc aagaaaagtt acaaattaca attaattgat tgctca
//