LOCUS EY194628 392 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab09c02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|P41042|RS4_DROME 40S ribosomal protein S4 pir|JN0871 ribosomal protein S4 - fruit fly (Drosophila melanogaster) dbj|BAA03786.1| ribosomal protein S4 [Drosophila melanogaster], mRNA sequence. ACCESSION EY194628 VERSION EY194628.1 GI:159498087 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 392) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 815 was extended to value 816 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 588 PolyA tail found inside read...SR being truncated to 544 Potential full insert read...vec-to-vec length: 464 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..392 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggacg tgaagttcga ctccggcaat ctctgcatga ttaccggagg ccgcaacatg 61 ggtcgagtgg gcatcatcgg acacagagaa cgccacccgg gatcgtttga cattgtgcac 121 atcaaagact tggccggaca ccagttcgcc actcgtgtga agaacgtgtt cattatcggc 181 aagggcgcaa ccccgttgat cagtctgccg gcggacaagg gcatcaagct ctcgattgca 241 gaggagcgcg acaagcgact ggcccagaaa gtgaaagcca gccactagac gagtggtgat 301 ggatgacttg gtgggtacac aaaaaatttg ttgatttgtt atcttgaacg aatacttgtg 361 ttcacattgg ctgataaaca cgatttctgt ta //