LOCUS       EY194626                 471 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab09b12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_494398.1| Putative nuclear protein of eukaryotic
            origin [Caenorhabditis elegans] gb|AAF60412.1| Hypothetical protein
            Y110A2AR.3a [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY194626
VERSION     EY194626.1  GI:159498085
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 662
            PolyA tail found inside read...SR being truncated to 622
            Potential full insert read...vec-to-vec length: 543
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcag accgcatttg tgggcatcgg cttttcggac cgcagcgact ccttcgactt
       61 gaatgtggca gttcaagatc atttcaaaac agtgaagaag tgcgaagagt catcagtgca
      121 ggaggaatcc aatcagccga agttggattt gagcttcaag gaaggacaaa ctatcaccgt
      181 taacattgga aaaaagcgca cctcctcgat gaataacgtc catttgacaa atggcagggc
      241 agtaccaact gctggtggcg gactcgtccc actattgcca ccaccgcctt catcgggaaa
      301 taaagtccga aaagggtgat attgccaatc ggaactgtga tacacgatgc caacaaagaa
      361 caaatccctc actccccaca ttgttttgtg tctttgatgt tctcataatc aaaagcgttt
      421 atggtttatt tttaaagccc aaatcacttc ccacaatgct aatagttacc a
//