LOCUS       EY194621                 340 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab09b06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194621
VERSION     EY194621.1  GI:159498073
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 811 was extended to value 811 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 533
            PolyA tail found inside read...SR being truncated to 492
            Potential full insert read...vec-to-vec length: 412
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ggcagccaaa ccctcgccat gcgcaaaacg ttcctcgcga cgccgttctt
       61 cacctattac acgcaggaga ctacagtgag catccagatc gcgactatta gcatcttctg
      121 tgactgcaat tgaggcgcaa gacagaagag agtggcgtcc aacggattcc tttctcgagg
      181 aagtcaccac gtgttgagcc gcaccttttc tctccagcac tttcctgtgc atagtcctaa
      241 aagaggcaag acatcgccct ttgacccaaa acgacagaac accgccgctt tttgtagttt
      301 tcttacactt tccttgtgaa taaatggtgc gtgagtccga
//