LOCUS EY194611 499 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab09a06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_503548.1| SNF2 helicase related protein family member [Caenorhabditis elegans] pir|G88961 protein F59A7.8 [imported] - Caenorhabditis elegans gb|AAB65339.1| Hypothetical protein F59A7.8 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY194611 VERSION EY194611.1 GI:159498053 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 499) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 830 was extended to value 831 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 834 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..499 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat tgtcgccgtg ctgagcgcat tagatcgagt tatggagtgt ggtgataaat 61 gtgtcattgt gagccagtgg acgaccatgt tgtcactgat cgagttccat ctgcaacgaa 121 agaagatcgg ctacaccagc attacggggg aggtgaagac cgatgaccgc cagtcgcgtg 181 tggacgcctt caaccgcgtt ggctctggac ccagagtgat gctgctctcg ctgactgtgg 241 ggggagttgg actgaacttg gtcggcggca atcatctctt cctggtcgat ctgcactgga 301 acccagcgct ggaacagcag gcctgtgacc gaatctaccg cctcgggcaa aagaaaagtg 361 tgttcatcca caaattcatt tgcaatgaga ccatcgagca acgcgtactg atgttgcagg 421 agaaaaagac cgcccttgcc acttccgtcc tggaaggtgc tgcctccacg aagattacta 481 agttgaccag ggatgagct //