LOCUS       EY194605                 278 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08h09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194605
VERSION     EY194605.1  GI:159498047
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 473
            PolyA tail found inside read...SR being truncated to 430
            Potential full insert read...vec-to-vec length: 350
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga aaaaagatac tcgactcttt tgtcaacaca aacatcctaa aacgggcggg
       61 aacagttgac gaaatcgcca atctgatcgt ctatttggcc agctccaagg cggcattcat
      121 cactggcacc accatggtca ttgacggagg ggccgtcgtt cgatcggagt tctgatctga
      181 gttcttcggc atattttacg gaaacagcct aactagtaac tactaatatt ttaataaaac
      241 atcctttgag aattcgctat aaataaatgt tcaaaata
//