LOCUS       EY194598                 594 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08h01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_705357.1| DNA topoisomerase III, putative
            [Plasmodium falciparum 3D7] emb|CAD52594.1| DNA topoisomerase III,
            putative [Plasmodium falciparum 3D7], mRNA sequence.
ACCESSION   EY194598
VERSION     EY194598.1  GI:159497105
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 803 was extended to value 803 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 806
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc cggagcaatt gcacgtgcat ttaaatggta caagcgagaa gccacaattg
       61 aactaaccga acgggaaata cgacgtcgcc gtaaagaaca cgaaaagaag aagaacccaa
      121 caagtgaaga accggacctc aatccagtga ggacatttgt ctacgaattc caaggcgtgt
      181 ttcaaagaca acaagcacag ttcaaagtca tcgccaccta tggaaatgtg acatccaccg
      241 cctttttgga agagtttgag cgacaggatg aagacgacga agattacaac tggcgcaaat
      301 acggttcgac gaaaaaattt cgcaagctat tcgatgttgg caggacgaaa aaggaaggcg
      361 ataaaacgaa agaaattcac aagttcatcg cgagtgaagc ggacgattgc cacgcaattg
      421 tgctctggtt ggactgcgac cgggcaggag aacacattgg gttcgaggtt atggaggttg
      481 tgcgcaaaaa atgggagcaa atagctgagc tgaacggcca acctgtgctg agcgacgagc
      541 aattcttgga acgtgtgttc agcgccaggc tgtgtttgag atgttatacc ccag
//