LOCUS       EY194589                 323 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194589
VERSION     EY194589.1  GI:159497067
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 373 (,)
            original QR value of 693 was extended to value 694 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 522
            PolyA tail found inside read...SR being truncated to 473
            Potential full insert read...vec-to-vec length: 395
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatt ttttgtaatt atgattttag gttataaata attaatgaag tataaactat
       61 taatgttata ttttttagat aaattaattt ttctattatt tttttttaaa ataaattatg
      121 gtcccaaaat tatcggcaaa acttttttat ttttaacaaa aaacttttta atttttattt
      181 tatttaatat aattttatat aaaaataggg aaaattttaa ttataattag gtaaataatt
      241 taaaaaaatt taaattttta ttttttacct taggtaaaac cctaattaag gaaatttatt
      301 atttttctta attaattttt gga
//