LOCUS EY194584 348 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab08f10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_509736.1| Papain family cysteine protease family member (45.7 kD) [Caenorhabditis elegans] pir|T24030 hypothetical protein R07E3.1 - Caenorhabditis elegans emb|CAA89070.1| Hypothetical protein R07E3.1 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY194584 VERSION EY194584.1 GI:159497062 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 348) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 540 PolyA tail found inside read...SR being truncated to 499 Potential full insert read...vec-to-vec length: 420 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..348 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga ccggtgaatg tcggaattgc cgtgcctccg gacatgaagc cctacaaatc 61 gggcatctac cacccttcgg actacgactg caaattcagg gtgctggggc tgcacgcatt 121 gctggtggtc ggctacggag ggagccagga aggcgtcaaa tactggatcg tgaagaacag 181 ttggaacaac acgtggggac aggagcacgg ctacgtgaac ttcgtccgtg gcatcaacgc 241 ctgcggaatc gaggacgagc caattgggct gttggcctga gggagcagtg agcattgcat 301 tttgtccacg cgattgttag tctcgaacac aaatgagacc aaatgaca //