LOCUS EY194582 266 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab08f08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194582 VERSION EY194582.1 GI:159497034 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 266) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 458 PolyA tail found inside read...SR being truncated to 416 Potential full insert read...vec-to-vec length: 338 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..266 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat atgtaatcgc aaaaaacggg atgaagcatt tgcgcagcta tgcagcggag 61 gcgtgtgcaa gacggcgagc gcgagtactg gcagaaagaa aagagagacc gcagtgtttg 121 aagcagcaaa acaaaaacgc aaacgtcatt tggctgtttt ggcagaaaac ctgaactgat 181 gtgagaaaca agaagtatga aatgaaaaaa tgatattaac acttttcatt cacatatatt 241 ttaaatcgta aaaaaaccaa taccga //