LOCUS       EY194580                 488 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194580
VERSION     EY194580.1  GI:159497032
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 823 was extended to value 824 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 682
            PolyA tail found inside read...SR being truncated to 639
            Potential full insert read...vec-to-vec length: 560
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc caaagaaagc aataaaacag caaagatgaa gcagtgcttc ttcttactct
       61 cctcgctgct actcgtcctc tgcttgctca ccaacggaca agtccccagc cagcccaact
      121 cgggcgagca acctgccggc caacagggcg gcccatctcc cccgcgtgga ccgcctccgt
      181 tctgcccgtc ctccgaccaa gcagtgcagg cgtgctgcga cgagttgcag gcgaatggac
      241 cggcatctgc cgaatcgggg ccgccagcgc cgccgcaaaa gtgcatcgcc tgcggagaga
      301 agtgcgcgcc gaagggacct ccaccggcat aaatgggaag gagtggacaa gaagtaattg
      361 acgcgaaaat ggaacaacta agtcgctctt tgtgagaaca accgctatct aaaatttctg
      421 tccccaatca ttcttaaatt cataataaat ttatgcggtg gatggtataa taaactctta
      481 tagagcca
//