LOCUS       EY194578                 495 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194578
VERSION     EY194578.1  GI:159497030
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 821 was extended to value 821 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 682
            PolyA tail found inside read...SR being truncated to 646
            Potential full insert read...vec-to-vec length: 567
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggct ctggctgagc atgtccgcca tcggaatgct catctggcac gccttgcgcc
       61 atctctacgc tcgacggctt gaacagaaca tggaaaaaca actctcgcac ttctcgtcct
      121 cgaaaaaaca accaacggaa atggcgaaga cggcagatgt cgggatcaat attgaaggaa
      181 acggcgaaaa attgccggaa aagatgttcc aagaaccttt tggagtcaac ttcatccagt
      241 tcccgctacc aatgccaccg ccttattctc caccggtgac cacattcagc caggagaagg
      301 aaatgacccc gtctcctccc gaacaactac tcgttgctgg aaacgggcag ttttgaagtg
      361 cacatcgaac cacgcttaag gcgtcacaga atacaaacaa aaaccttgat cggaactcgt
      421 tgttgaaaat tcatttatca aaaacgataa aaatgaatga cgcatattga tgtgaaaaag
      481 tataaaatgt gtgga
//