LOCUS       EY194576                 359 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194576
VERSION     EY194576.1  GI:159497028
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 824 was extended to value 825 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 553
            PolyA tail found inside read...SR being truncated to 510
            Potential full insert read...vec-to-vec length: 431
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga ggagggacca ctttcgccgg aacaagcgaa cgagttccta cagctgttgc
       61 gaatgcaaca acagcgctcg gccaacgccc cgcagtacgc gcagtgagat gggaaaggag
      121 cggaacggag aggactcgct gcacaaacat tccgagaaac gatctcccgc ccgaccctaa
      181 cgatggatga atgccctccc cacccccaat cttcattcct caacgactac gcgtgacccg
      241 gttccccaca atgtagattc tctcccacaa ctgcttgccc tgccctattt atgtttgtac
      301 tggcaatgcg gcggcacacg acgaagacag acggcggcga ataaacagtg ttgaccaca
//