LOCUS       EY194574                 407 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08e12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194574
VERSION     EY194574.1  GI:159497026
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 600
            PolyA tail found inside read...SR being truncated to 558
            Potential full insert read...vec-to-vec length: 479
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag cacgatcacc aatggcctca gaaaggccgc cagtttcttg catatcactt
       61 cgccaaagaa agacaagtcc aaggagaaga agggttcggc aaagccaaag gagcagaagg
      121 cgaaggcgtc gcctaaagca gctccacatg acccaaaagc gtcgcctaaa acggctctac
      181 atccacatga cccaaaggca acggttccta aggcctaaac atgcgtcgta atttttgcgc
      241 gcagaaataa attcatgtct tcacgtgtga ttttgtgccc aaacgacgct gcattgttgt
      301 gttatgttca aacttcaaat ttgtgttcaa aaagctttcc tgtctttttt cgtcattttc
      361 ggggtcattt tttgtgcatc aaagcgataa agttggtcac aacttca
//