LOCUS       EY194566                 243 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pir|E81737 hypothetical protein TC0128 [imported] -
            Chlamydia muridarum (strain Nigg), mRNA sequence.
ACCESSION   EY194566
VERSION     EY194566.1  GI:159496988
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 830 was extended to value 834 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 437
            PolyA tail found inside read...SR being truncated to 394
            Potential full insert read...vec-to-vec length: 315
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtct gcatatgtta catatagtca ggtaagtaaa ttataaagag cacttgatgg
       61 atgccttggc gttaagaggc gatgaaggac gtggcaggct gcgataagct gcggggaact
      121 gtcaacaagt tttgatccgc agatttccga atggggaaac ccaactagct tagctagtta
      181 ttttatgctg tataagtatg aaaggtaaac ttggtgaact gaaatatctc agtagccaaa
      241 gga
//