LOCUS EY194561 594 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab08d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_505451.1| Ligase family member (60.3 kD) [Caenorhabditis elegans] pir|T20741 hypothetical protein F11A3.1 - Caenorhabditis elegans emb|CAA94751.1| Hypothetical protein F11A3.1 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY194561 VERSION EY194561.1 GI:159496954 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 594) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 807 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..594 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa ggtcaaagtg gcgatgttag ttccgcccat cctcgttctc ctctcaaagt 61 cgcaatcagt gatttcgcgc tacgacctct cttcactgcg cgttctcatg accggagctg 121 caccagccgg ccacgatttg ctggaggaag tgaagcgccg actgccttcc gtggagcaca 181 tctgccaggg ctacggaatg acggaacagt ccatgtgttc gcatttgcca gtttttggca 241 tgctgaaccc aaacgcggca gggagactca tttccaactt cgaaatgaag attgtctgta 301 cggaaacagg aaggccagtt gaggcggggc aaccaggcga gatttgtacc cggagtccaa 361 ccactatgct tggttacttg aattgcccaa aagcgaccat ggagaccgtg gacaaggatg 421 ggtggcttag aactggcgac atcggctatt ttgacgacaa gggctggact tatgtggtgg 481 agaggctgaa agagctgatc aaagtcaacg ggcttcaggt ggcaccagcc gaactagagg 541 atttgatttt gtcgcacccg cacgtacaag actgcgctgt gataggcgtt ccgg //